the main relaxant constituents of nigella sativa methanolic fraction on guinea pig tracheal chains.
Authors
abstract
our previous studies demonstrated the preventive effect of nigella sativa extract on asthma and water-soluble substances of this extract, especially its methanol fraction were responsible for this relaxation on contracted tracheal chains of guinea pigs. for the first time, the present study has been conducted to determine the main constituents of its methanolic extract. four constituents of 20%-methanolic fraction, consisting of two flavonoids (20-20% and 21-20% fractions) and two polysaccharides (1-20% and 2-20% fractions), were purified by analytical and preparative hplc. the relaxant effects of four cumulative concentrations of each constituent (50, 100, 150 and 200 mg/lit) in comparison with saline (1 ml) as negative control and four cumulative concentrations of theophylline (0.2, 0.4, 0.6 and 0.8 mm) as positive control were examined on methacholine-precontracted guinea pig tracheal chains (n=6). all concentrations of theophylline and most concentrations of 20-20, 21-20 fractions showed significant relaxant effects compared to that of saline. 20-20 fraction (comferol diglucoside) was the most potent bronchodilator. their relaxant effects were lower than that of theophylline. polysaccharides (1-20, 2-20 fractions) did not have any relaxant effects compared to that of saline. these results revealed that two flavonoids of 20%-methanolic fraction of nigella sativa were its main relaxant constituents.
similar resources
The Relaxant Effects of Different Methanolic Fractions of Nigella sativa on Guinea Pig Tracheal Chains
Objective(s): In regard to the high incidence of asthma and the side-effects of the drugs used, finding novel treatments for this disease is necessary. Our previous studies demonstrated the preventive effect of Nigella sativa extract on ovalbumin-induced asthma. In addition, water-soluble substances of N. sativa extract and methanol fraction of this plant were responsible for the relaxant eff...
full textThe Relaxant Effects of Different Methanolic Fractions of Nigella sativa on Guinea Pig Tracheal Chains
OBJECTIVE(S) In regard to the high incidence of asthma and the side-effects of the drugs used, finding novel treatments for this disease is necessary. Our previous studies demonstrated the preventive effect of Nigella sativa extract on ovalbumin-induced asthma. In addition, water-soluble substances of N. sativa extract and methanol fraction of this plant were responsible for the relaxant effect...
full textthe relaxant effects of different methanolic fractions of nigella sativa on guinea pig tracheal chains
objective(s): in regard to the high incidence of asthma and the side-effects of the drugs used, finding novel treatments for this disease is necessary. our previous studies demonstrated the preventive effect of nigella sativa extract on ovalbumin-induced asthma. in addition, water-soluble substances of n. sativa extract and methanol fraction of this plant were responsible for the relaxant effec...
full textPossible Mechanism(s) of the Relaxant Effects of Achillea wilhelmsii on Guinea-Pig Tracheal Chains
Achillea wilhelmsii have been used in folk remedies. The relaxant effects of the extractof A. wilhelmsii on tracheal chains of guinea pigs were examined. The relaxant effects offour cumulative concentrations of the extract, theophylline and saline were examined bytheir relaxant effects on precontracted tracheal chains of guinea pig by KCl (group 1), 10 μMmethacholine (group 2), incubated tissue...
full textPossible Mechanism(s) of the Relaxant Effects of Achillea wilhelmsii on Guinea-Pig Tracheal Chains
Achillea wilhelmsii have been used in folk remedies. The relaxant effects of the extractof A. wilhelmsii on tracheal chains of guinea pigs were examined. The relaxant effects offour cumulative concentrations of the extract, theophylline and saline were examined bytheir relaxant effects on precontracted tracheal chains of guinea pig by KCl (group 1), 10 μMmethacholine (group 2), incubated tissue...
full textPossible mechanism(s) for relaxant effect of aqueous and macerated extracts from Nigella sativa on tracheal chains of guinea pig
BACKGROUND In previous studies, the relaxant, anticholinergic (functional antagonism) and antihistaminic effects of Nigella sativa have been demonstrated on guinea pig tracheal chains. To elucidate the other mechanisms responsible for the relaxant effect of this plant, its inhibitory effect on the calcium channel was examined in this study. RESULTS The inhibitory effects of both concentration...
full textMy Resources
Save resource for easier access later
Journal title:
iranian journal of allergy, asthma and immunologyجلد ۱۲، شماره ۲، صفحات ۱۳۶-۱۴۳
Hosted on Doprax cloud platform doprax.com
copyright © 2015-2023